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Inside the healthy donor tissues, T-bet phrase try caused once 18 occasions from stimulation, and you may maximum T-bet protein membership would be sensed after twenty four in order to a couple of days that have PMA and you can ionomycin. STAT1 offered since the a control to have variations in cytoplasmic-atomic localization ; a polyclonal antibody facing actin was utilized to exhibit equivalent loading of the examples. Citește mai mult